Effect of (2-chloro-4-pyridyl)-N-phenylurea (CPPU) on artemisinin production in hairy root culture of Artemisia annua L

Abstract

-1 (4 µM), 2.5 mg L -1 (10 μM) and 5 mg L -1 (20 μM) in the form of (2-chloro-4-pyridyl)-N-phenylurea (CPPU) was applied to culture media after 10 days of subculture. Artemisinin content was measured at different exposure periods (0, 24, 48, and 72 h). Our result shows that the highest artemisinin content was observed from hairy root that was treated with 5 mg L -1 (20 μM) of CPPU for 72 h which were about 3 folds higher than that of non-treated culture.