Effect of 2,4-D and BAP on the Saurauia bracteosa In Vitro Culture

Abstract

Saurauia bracteosa DC. (Pirdot) is one of the endemic woody plant species that has medicinal properties. The declining population in natural habitats caused this plant as a vulnerable species that need in vitro culture to prevent further extinction. The combination of 2.4-D and BAP as an exogenous PGR influenced the success of woody plant in vitro culture. Therefore, this research aimed to determine the effect of 2.4-D and BAP concentrations on the in vitro response of the S. bracteosa leaf explant. This research used a complete randomized design (CRD) with two factors: (1) the concentration of 2.4-D (0.50; 0.75; 1 mg/L), and (2) the concentration of BAP (B) (1; 2; 3 mg/L). This research consisted of 16 treatments (including a control); each treatment was repeated three times. All data were analyzed statistically using ANOVA, and the significant results will be followed by Duncan’s Multiple Range Test (DMRT). All PGR treatments could not produce a callus growth response, yet all explants showed various responses to browning intensity (0.08–1) and 100% browning. Further research still needs to be done to find the best type of explant and concentrations of PGR that can overcome the recalcitrant explant of Sauraia bracteosa. These results can be valuable information to promote the successful in vitro conservation of Sauraia bracteosa.