Effect of 1-naphthylacetic and indol-3-acetics acid on the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro

Abstract

Aim. Investigate the effect of auxins of exogenous origin in nutrient medium in vitro on the germination and organogenesis intensity in Linum usitatissimum L. convar. elongatum (‘Hlinum’ variety) at the constant concentration of 6-benzylaminopurine (BAP). Methods. Hypocotyl segments were cultured on Murashige and Skoog nutrient medium supplemented with sucrose (30 g/l) and phytohormones at various concentrations. Other conditions: photoperiod 16 hours, relative humidity 60–80%, air temperature 22–24°C. Results. Common flax has a great capacity to form callus and shoots under the effect of the following factors: 1) only auxins, 2) only cytokines, 3) combinations of auxins and cytokines. Somatic embryogenesis is also possible on a nonhormonal nutrient medium. Conclusions. For somatic embryogenesis in vitro, the optimal concentrations of BAP can be expressed as 1.0 ≤ BAP ≤ 1.75, the optimal concentrations of BAP for the medium supplemented with 1-naphthylacetic (NAA, 0.05 mg/l) 0.5 ≤ BAP ≤ 2.0, the optimal concentration of NAA for the medium supplemented with BAP (1.0 mg/l) 0.025 ≤ NAA ≤ 0.150, and the optimal concentrations of indol-3-acetics acid (IAA) for the medium supplemented with BAP (1.0 mg/l) 0.05 ≤ IAA ≤ 0.50. Keywords: Linum usitatissimum L., in vitro, phytohormones, callus, organogenesis.