Abstract
1. 1. Pretreatment with 1,25-dihydroxy vitamin D 3 [1,25-(OH) 2D 3] caused an increase in omithine decarboxylase (EC 4.1.1.17) (ODC) activity in lipopolysaccharide (LPS)-stimulated human monocyte cell line, U937. 2. 2. The increase in ODC activity was dose-dependent and preincubation-time dependent. 3. 3. 26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D, was ca 7 times more potent than 1,25-(OH) 2D 3 in increasing ODC activity. 4. 4. Pretreatment with 1,25-(OH) 2D 3 also potentiated the activity of spennidine/spermine- N 1-acetyltransferase in LPS-stimulated U937 cells. 5. 5. Putrescine levels in cells pretreated with 1,25-(OH) 2D 3 increased ca 2-fold 4–8 hr after LPS addition. 6. 6. However, pretreatment with 1,25-(OH) 2D 3 did not cause any increase in ODC mRNA level, suggesting that 1,25-(OH) 2D 3 may modulate polyamine metabolism at the posttranscriptional level rather than the transcriptional step.
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