Abstract
Abstract The present study was undertaken to examine effect of γ-rays on the antioxidant and antiproliferative potential of β-glucan isolated from oats. Irradiation doses of 0, 2, 6 and 10 kGy were given to extracted β-glucan. The samples were characterized by Fourier transform infrared spectroscopy, Gel permeation chromatography and quantitative estimation by (1-3) (1-4) β- d -Glucan assay kit (Megazyme). The average molecular weight of non-irradiated β-glucan was 199 kDa that decreased to 70 kDa at 10 kGy. Both FT-IR spectrum and chemical analysis revealed that the extracted β-glucan was pure having minor impurities. Antioxidant activity was evaluated by DPPH, lipid peroxidation, reducing power, metal chelating ability and oxidative DNA damage assays. Results revealed increased antioxidant activity of β-glucan with the increase in irradiation dose. Irradiated β-glucan also exhibited dose dependent cancer cell growth inhibition with irradiation doses. The study revealed that the low molecular weight β-glucan with increased antioxidant and antiproliferative activities can be produced by irradiation treatment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
