Abstract
High fat diet feeding results in hyperglycemia and insulin resistance, which is a major pathological feature of type-2 diabetes mellitus. The use of oral hypoglycaemic drugs is limited due to its deleterious side effects and there is a need to find more efficacious agents for diabetes management. Hence, it is of interest to show the mechanism of action of β-Caryophyllene on insulin signalling molecules in gastrocnemius muscle of high fat diet - induced type-2 diabetic rats. An oral effective dose of with β-Caryophyllene (200 mg/kg b.wt) was given for 30 days to high fat diet (comprising 2% cholesterol, 1% cholic acid, 30% coconut oil, 67% conventional rat feed) and fructose fed type-2 diabetic rats to find out whether β-Caryophyllene regulates IRS-1/Akt pathway of insulin signalling. The data shows that, β-Caryophyllene treatment significantly increased the mRNA and protein expression of insulin receptor (IR) in diabetic rats whereas there is no significant difference in mRNA expression of insulin receptor-substrate-1 (IRS-1) was observed among groups. The Akt mRNAand GLUT-4mRNA and protein level were also improved in gastrocnemius muscle of type-2 diabetic rats. Thus, we concluded that β-Caryophyllene could be used as potential phyto medicine for type-2 diabetes management.
Highlights
Type-2 diabetes is a progressive condition in which the body grows resistant to the usual impact of insulin and/or gradually loses the ability of the pancreas to produce enough insulin [1].Western-style diets, low in dietary fiber and high in saturated fatty acids, are implicated in increased risk of diabetes and obesity [2]
Mechanism for the sustainability of glucose homeostasis is the rapid action of insulin to stimulate the uptake and metabolism of Experimental design: glucose in tissues [4]
Skeletal muscle and liver were the main The following experimental design was framed and sites of glucose elimination in the insulin-stimulated state and it the rats were subjected to treatment for a period of one month
Summary
Declaration on Publication Ethics: The author’s state that they adhere with COPE guidelines on publishing ethics as described elsewhere at https://publicationethics.org/. The authors undertake that they are not associated with any other third party (governmental or non-governmental agencies) linking with any form of unethical issues connecting to this publication. The authors declare that they are not withholding any information that is misleading to the publisher in regard to this article. Author responsibility: The authors are responsible for the content of this article. The editorial and the publisher have taken reasonable steps to check the content of the article in accordance to publishing ethics with adequate peer reviews deposited at PUBLONS. Declaration on official E-mail: The corresponding author declares that official e-mail from their institution is not available for all authors
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