Effect Fermentation of Sugarcane Shoots With Phanerochaetechrysosoporiumon the Activity of Lacase Enzymes, Lignin Peroxidase and Manganese Peroxidase

Abstract

Sugarcane shoots are very potential for make feed because there are quite a lot of them. Efforts to use sugarcane shoots by products have been carried out but it is still not optimal. One strategy that can be done is to use it as fermented feed. Main constraints on use of sugarcane shoots for animal feed are nutritional value, low digestibility and high lignin. Lignin physically and chemically is a major factor in the inability of livestock to digest feed ingredients. Fungi that have the ability to degrade high lignin are fungi which are included in the white rot fungi. One of them is Phanerochaetechrysosporium which is known to produce enzyme lignin peroxidase (LiP), manganese peroxidase (MnP) and lacase. Phanerochaetechrysosporium is one of the fungi could break down the bonds and degradate lignin with the help of lignin degrading enzymes. In this study sugarcane shoots were fermented using Phanerochaetechrysosporium. This method study used Completely Randomized Design (CRD) with 4 treatments inoculum dose A (0%), B (5%), C (10%), D (15%) with 5 replications. Data processing by analysis of variance (ANOVA), if it was significantly different in further testing with DMRT. Results showed that treatment C (10%) was the best dose that could be used with the production of lacase enzyme activity 2.016 U / ml, Lignin Peroxidase (LiP) 1.677 U / ml and Manganese peroxidase (MnP) 0.328 U / ml.