Abstract
Objective To establish an accurate sorting method with less interference to the cell activity for isolating CD4+T lymphocytes.Methods Human peripheral blood cells were sorted by density gradient centrifugation (DGC) and magnetic activated cell sorting (MACS) in turn,and then the cell purity,morphological observation and survival rate of CD4+T lymphocytes were adopted to conduct the evaluation on the sorting method.Results The purity of CD4+T lymphocytes was (38.8 ± 2.7)％,while the purity of CD4+T lymphocytes was (96.2 ± 0.7)％ after magnetic activated cells being sorted,the difference between them was statistically significant (P ＜ 0.01).The activity was the highest within 24 hours with intact cell shape and function.Conclusion The Percoll DGC combined with the MACS can collect highly pure CD4+T lymphocytes with less influence on the cell activity and shape,the sorted cells can continue to be used for related researches. Key words: CD4+T lymphocytes; Cell sorting; Magnetic-activated cell sorting
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