Effect Evaluation of Nucleic Acid Test Applied in Donator's Blood Screening

Abstract

Objective To explore the role of nucleic acid test (NAT) in screening the window period or occult hepatitis B virus infection (OBI) blood donators by comparing the results of NAT and enzyme-linked immunosorbent assay (ELISA) of the blood samples.Methods Form July to December 2011,A total of 13 126 blood samples from volunteer blood donors in Foshan blood center were included in this study.All of this samples were tested by NAT using procleix TIGRIS system and ELISA simultaneously.Five markers of hepatitis B virus (HBV) of donators who were positive tested by ProcleixUltrio and Discrimination but negative tested by ELISA were detected.Results Among all the samples,128 (0.98％) cases were NAT positive.Among the 128 cases,95 cases were detected positive by two kinds of ELISA reagents,4 cases were detected positive by only one kind of ELISA reagent,and another 29 cases were detected negative by two kinds of ELISA reagents.8 cases results were traced,and 6 of the 8 donators were detected the five markers of HBV,4 of them were positive for anti-HBc.Conclusions As a new detect method different from ELISA,NAT is used to cut down the window periods of virus detection.The blood screening strategy using both NAT and ELISA could improve the safety of blood transfusion. Key words: volunteer blood donors; nucleic acid test; enzyme-linked immunosorbent assay