Effect effects of &lt;i&gt;Auricularia auricula&lt;/i&gt; polysaccharides on exhaustive swimming exercise-induced oxidative stress in mice

H Hao

doi:10.4314/tjpr.v13i11.11

Abstract

Purpose: To determine the effects of polysaccharides from Auricularia auricula (AAPs) on exerciseinduced oxidative stress in mice.Methods: The animals were divided into four groups: control (C), low, middle, and high-dose AAPstreated (LA, MA, and HA, respectively). C group received physiological saline solution while AAPstreated groups received different doses of AAPs (50, 100, and 200 mg/kg) via oral gavage once a day for 28 days, followed by an exhaustive swimming exercise. Swimming times were recorded for each animal after which malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were determined.Results: Exhaustive swimming times were significantly prolonged in all AAPs-treated animals as compared with controls. In all treatment groups, serum and muscle MDA levels were significantly lower than those in the C group, whereas in the MA- and HA-treated groups, a decrease was also seen for liver MDA levels. Furthermore, the concentration of 8-OHdG was significantly reduced in serum, liver, and muscle in all AAPs-treated animals. In contrast, all AAPs treatment groups exhibited significantly higher SOD activity in serum, liver, and muscle. Serum and liver GPx activity was also significantly increased in all animals receiving AAPs treatment, with MA- and HA-treated mice exhibiting an additional augmented GPx activity in muscle. Serum and liver catalase activities in the MA and HAtreated groups, and catalase activity in muscle in all AAPs treatment cohorts, were significantly higher.Conclusion: Polysaccharides from Auricularia auricula enhance exercise endurance and possess protective effects against exhaustive swimming exercise-induced oxidative stress in mice.Keywords: Exhaustive swimming, Malondialdehyde, 8-Hydroxydeoxyguanosine, Superoxide dismutase, Glutathione peroxidase, Catalase

Highlights

Edible mushrooms have been recognized as important food items since ancient times and their consumption is increasing because of their significant role in human health, nutrition, and disease [1]
The aim of the present study was to determine the effects of AAPs on exercise-induced oxidative stress by measuring MDA and 8-hydroxydeoxyguanosine (8-OHdG) levels and the activities of the main antioxidant enzyme in the blood, liver, and muscle of mice
The exhaustive swimming times in the LA, MA, and HA groups (517.3 ± 75.3, 608.9 ± 62.7, and 783.8 ± 84.9 sec, respectively) were significantly higher than that in the C group (379.6 ± 48.9 s) (p < 0.05). These results suggest that AAPs have anti-fatigue activity and could enhance exercise endurance

Summary

Introduction

Edible mushrooms have been recognized as important food items since ancient times and their consumption is increasing because of their significant role in human health, nutrition, and disease [1]. Auricularia auricula, known as “black tree ear,” a precious macrofungus, is the fourth most important cultivated mushroom used by humans throughout the world [2] and has a history as traditional medicinal application in China. Polysaccharides have been identified as one of the main bioactive components of Auricularia auricula. The main monosaccharide That make up the polysaccharides from Auricularia auricula (AAPs) are glucose (72 %), mannose (8 %), xylose (10 %), and fucose (10 %). The aim of the present study was to determine the effects of AAPs on exercise-induced oxidative stress by measuring MDA and 8-hydroxydeoxyguanosine (8-OHdG) levels and the activities of the main antioxidant enzyme in the blood, liver, and muscle of mice

Objectives

Results

Conclusion