Abstract
Ectopic pregnancy (EP) is associated with significant morbidity and mortality, but the molecular mechanism of this condition is still unclear. miR-196b, a hot research direction for the past few years, participates in the occurrence of various diseases but whether it plays a regulatory role in EP is still unclear. This research was set to investigate the expression and potential value of miR-196b in EP. qRT-PCR was utilized to determine the relative expression of miR-196b in peripheral blood of EP patients and to observe the expression changes of miR-196b before and after treatment. Correlation analysis of miR-196b with HCG and progesterone was performed. Logistic regression analysis was applied to independent risk factors affecting EP patients. TargetScan was utilized to predict the downstream target genes of miR-196b, and GO and KEGG analysis was carried out using the R language pack. qRT-PCR showed that miR-196b expression in peripheral blood of EP patients was lower than that of normal people. miR-196b expression in patients after treatment was notably higher than that before treatment. In addition, correlation analysis showed that miR-196b was positively correlated with the expression of HCG, progesterone, and estradiol. Risk factor analysis revealed that abortion history, pelvic inflammatory disease history, lower abdominal surgery history, and miR-196b were independent risk factors for EP, and the AUC of the combined ROC curve was 0.899. GO function enrichment and KEGG signal pathway enrichment found 10 potential functions and 2 potential signal pathways of miR-196b. miR-196b is expressed in EP patients, is differentially expressed according to the change in EP condition, and is expected to become a promising index for clinical diagnosis of EP.
Highlights
Pregnancy is a unique clinical situation as it can be accompanied by special diseases [1]
89 Ectopic pregnancy (EP) patients treated in our hospital from January 2017 to January 2020 were included in the observation group (OG), and 70 healthy pregnant women who underwent physical examination during the same period were included in the control group (CG). e inclusion criteria of EP patients include the following: patients showed symptoms of vaginal bleeding and abdominal pain in the early pregnancy and were diagnosed as EP by ultrasound examination
All human chorionic gonadotropin (hCG) and progesterone measurements were performed in our hospital using commercially available analysis methods
Summary
Pregnancy is a unique clinical situation as it can be accompanied by special diseases [1]. Ectopic pregnancy (EP) is a complication of early pregnancy, in which the fertilized eggs are planted anywhere outside the uterine cavity without entering the uterus. Fallopian tube EP is the most common clinically [2]. The clinical diagnosis of EP is mainly made by ultrasound combined with serum human chorionic gonadotropin (hCG)/progestogen [3]. With continuous serum hCG measurement, the patient is at risk of tubal rupture during the delay before the hCG assessment [4]. The use of serum hCG and/or progesterone to determine EP is limited by false negative and false positive rates [5]. We hope to find new potential markers of early EP to solve this problem
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