Effect and mechanism of Vav3 gene to regulate inhibitor of apoptosis proteins in human gastri cancer cell line BGC823

Abstract

Objective To examine the expression of Vav3 in gastric cancer and the effect of inhibiting Vav3 gene on apoptosis of human gastri cancer cell line BGC823 cells and the the underlying mechanism. Methods Specific small interfering RNA(siRNA)targeting Vav3 was designed, synthesized, and transfected into BGC823 cells. The expression of Vav3 was detected by real- time quantitative polymerase chain reaction (Real- time PCR) and Western blotting. Cellular viability was measured by methyl thiazol tetrazolium (MTT)assay. Cell apoptosis rate was determined by flow cytometry(FCM). The expression levels of Survivin, c- inhibitor of apoptosis protein 1(IAP1), c- IAP2, XIAP, Livin and Cysteinyl aspartate- specific protease(Caspase)- 3 were also determined. Results As compared with para- carcinoma tissues(0.173± 0.025)and gastric epithelial cell line GES- 1(0.250±0.036), Vav3 level was significantly up- regulated in gastric cancer tissues(0.727±0.101)and gastric cancer cell line BGC823(0.850±0.140,P 0.05).The expression and activity of Caspase-3 were increased after Vav3- siRNA tansfection into BGC823 cells(all P< 0.05). Conclusion Vav3 was overexpressed in gastric cancer. Vav3 gene silencing effectively promoted gastric cancer cell apoptosis, suggesting that inhibiting Vav3 can promot apoptosis of gastric cancer cells by regulating some IAPs. Key words: Stomach cancer; Vav3; Small interfering RNA; Inhibitor of apoptosis proteins; Cysteinyl aspartate-specific protease-3