Abstract
Superovulation is a necessary technique to produce large number of embryos for embryo` transfer. Hormonal treatment is superovulation methode can be done by implant CIDR and injection of Follicle Stimulating Hormone (FSH). Experiment were carried out to observe the effectiveness of subcutaneous and intramuscular FSH injections on superovulation response in simmental cattle. All animal (n=10) were treated with intravaginal CIDR implant before FSH injection. Studies were devided into two experiment ie: P1 (400 mg FSH diluted in 4 ml sterile diluent) injected in five simmental cattle by single subcutan injection and P2 (400 mg FSH diluted in 20 ml sterile diluent) injected in five simmental cattle by twice daily intramuscular injection over 4 day in decreasing doses. The number of corposa luteal (CL), total embryos collected, and total transferable embryos were observed in this experiment. Data were analyzed by T-test method. The result showed that effectiveness of single subcutan FSH injection were significanthy different (P < 0,05) than intramuscular FSH injection superovulation with single subcutan FSH injetcion is easier than twice daily intramuscular injection in decreasy dose. In conclusion the average of CL (21,4 ± 3,6) and number of tranferable embryo (71,96 %) of the single subcutan FSH injection tended to be better than intramuscular FSH injection. Single subcutan FSH injection more efficient than intramuscular FSH injection. Single subcutan injection can decreasing sterss level in the cattle and be easier in handling the cattle during the experiment.Keywords: Simmental cattle, FSH, superovulation, single subcutan injection, intramuscular injection.
Highlights
ABSTRAK Superovulasi merupakan salah satu tahapan penting untuk dapat memproduksi embrio dalam program transfer embrio
Studies were devided into two experiment ie: P1 (400 mg Follicle Stimulating Hormone (FSH) diluted in 4 ml sterile diluent) injected in five simmental cattle by single subcutan injection and P2 (400 mg FSH diluted in 20 ml sterile diluent) injected in five simmental cattle by twice daily intramuscular injection over 4 day in decreasing doses
The result showed that effectiveness of single subcutan FSH injection were significanthy different (P < 0,05) than intramuscular FSH injection superovulation with single subcutan FSH injetcion is easier than twice daily intramuscular injection in decreasy dose
Summary
Penelitian dilaksanakan bulan April sampai Mei 2017 di Balai Embrio Ternak Cipelang Bogor. Alat penelitian sesuai tahapan pelaksanaan, yaitu: superovulasi dengan menggunakan alat: spuit berukuran 5 ml dengan jarum suntik berukuran 18 dan 22 G untuk penyuntikan FSH dan PGF2α, sarung tangan dan tambang. Koleksi embrio menggunakan alat: servix expander (pembuka lumen servix), inner stilet, selang silicon dengan Y-konektor, folley catheter, spuit 50 ml untuk spul (desinfeksi saluran reproduksi) dengan iodine povidon, spuit 5 ml untuk anastesi epidural, spuit 20 ml untuk fiksasi balon kateter, botol media 500 ml untuk penampungan media flushing, infusion tube, plastic glove, jarum ukuran 18 G, dan gun spul. Untuk melihat respon superovulasi pada penelitian ini sapi donor diberi 2 perlakuan yaitu: P1 = penyuntikan FSH sebanyak 1 kali secara subcutan pada hari ke-4 dengan dosis 400 mg dengan campuran cairannya
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