Abstract
Background. The incidence of Multi Drug Resistant (MDR) in extraintestinal E. coli has become a global problem in the world. In Indonesia, the greatest resistance to the uropathogen E. coli is resistant to ampicillin (91.9%), ciprofloxacin (83.7%) and cefixime (67.6%). Therefore it takes effort for the treatment of MDR uropathogen E. coli, one of them are development of new antibiotics from herbal isolates of Garcinia mangostana L., a-mangosteen. This study examined the activity of a-mangosteen in vitro on the growth of MDR uropathogen E. coli.Methods. Treatment of uropathogen E. coli is performed in vitro, using a-mangosteen with a range of levels 14 - 450 �g/mL. The antibacterial activity of a-mangosteen is measured by determine at the growth or death of bacteria at each concentration using indirect methods, which is absorbance reading. Uropathogen E. coli that had been treated with various concentration of a-mangosteen incubated for 18-20 hours, then read an absorbance at wavelenght 625 nm using a spectrophotometer.Result. The Minimal Inhibitory Concentration (MIC) of a-mangosteen in this study was 450 �g/mL. Based on the linear regression (STATA 13.1) relationship between concentration of a-mangosteen and activity of growth inhibition of bacteria, obtained the F test value 0.0001 < 0.05, states that all concentrations of a-mangosteen simultaneously have a significant influence on the growth of uropathogen E. coli.Conclusion. MIC value is relatively large causing a-mangosteen activity against Gram-negative bacteria needs to be studied further. Potentially relevant activity in the clinic will occur if the value of in vitro MIC < 100 �g/mL. Likewise, the pharmaceutical industry prefers the development of antibiotics that have in vitro MIC values = 2 �g/mL.
Highlights
Introduction: In Indonesia, the most commom uropatogen E. coli resistance has been to ampicillin (91.9%), ciprofloxacin (83.7%) and cefixime (67.6%). α-mangostin, a chemical compound, has been developed as a new antibiotics isolaated from herbal Garcinia mangostana L, but its effectiveness against multiresistant uropathogenic E
This study examined the effect of α-mangostin on growth of multiresistant E. coli Methods: α-mangostin Treatment of E. coli uropatogen bacteria was administered in vitro, using 14 levels of concentration 14; 28,13; 56.25; 112.5; 225; And 450 μg/mL with 4 times replication at each concentration
Agar media of Eosin Methylene Blue (EMB) was used to distinguish E. coli lactose fermentant from Enterobacter aerogenes (Oxoid, 2010). biochemical tests, ie indol test, methyl red, proskaur voges, and citrate were done for confirmation
Summary
In Indonesia, the most commom uropatogen E. coli resistance has been to ampicillin (91.9%), ciprofloxacin (83.7%) and cefixime (67.6%). α-mangostin, a chemical compound, has been developed as a new antibiotics isolaated from herbal Garcinia mangostana L, but its effectiveness against multiresistant uropathogenic E. Objective: This study examined the effect of α-mangostin on growth of multiresistant E. coli Methods: α-mangostin Treatment of E. coli uropatogen bacteria was administered in vitro, using 14 levels of concentration 14; 28,13; 56.25; 112.5; 225; And 450 μg/mL with 4 times replication at each concentration. The most common extraintestinal infections caused by the bacteria of Escherichia coli (E. coli) has been urinary tract infection (UTI). This disease occurs at least 25-35% adul women during their life (Sukandar, 2009). The incidence of multi-drug-resistant uropathogenic Escherichia coli has been reported in Nepal (Baral et al, 2012) represeting 38.2% total isolates of patient with UTI.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
