Abstract
Quality of biofertilizer such as arbuscular myorrhizal fungi could be improved through the selection of appropriate sterilization and composition of the medium. The objective of the experiment was to determine the effect of sterilization and carrier composition on root colonisation, root length and weight of sorghum root. A green house experiment was conducted in randomized block design. The factors were: 1. methods of sterilization (ie. control; autoclave; NaOCl 10%; Co-60 gamma irradiation), and 2. composition of the carrier of mycorrhiza (100% zeolite; 75% zeolite + 25% rice charcoal; 50% zeolite + 50% rice charcoal). Results showed that there was no interaction effect of sterilization and composition of carrier on root colonisation, root length and weight of sorghum root. However, different technique of sterilisation gave differences on root colonisation, root length and weight of sorghum root. Sterilization using NaOCl 10% was not recommended. Gamma Co-60 irradiation sterilization did not have difference with control and autoclave, however there was tendency that irradiation technique gave better results to all parameters measured.
Highlights
Results showed that there was no interaction effect of sterilization and composition of carrier on root colonisation, root length and weight of sorghum root
such as arbuscular myorrhizal fungi could be improved through the selection of appropriate sterilization and composition
The objective of the experiment was to determine the effect of sterilization and carrier composition on root colonisation
Summary
EFFECT OF STERILIZATION AND MEDIUM COMPOSITION FOR MYCORRHIZAL FUNGI ON ROOT COLONISATION, LENGTH AND WEIGHT OF SORGHUM ROOT. Pengujian kualitas inokulan mikoriza yang berkualitas antara lain dapat dilakukan melalui optimasi metode sterilisasi dan komposisi media. Penelitian yang bertujuan untuk mengetahui pengaruh sterilisasi dan komposisi media terhadap derajat infeksi akar, panjang akar, dan bobot kering akar sorgum telah dilakukan di rumah kaca. Hasil penelitian menunjukkan bahwa tidak terdapat interaksi antara perlakuan sterilisasi dan komposisi media tanam terhadap peningkatan kolonisasi akar, panjang akar, dan bobot akar sorgum. Teknik sterilisasi media berpengaruh terhadap kolonisasi akar, panjang akar dan bobot akar sorgum. Teknik sterilisasi dengan autoklaf dan iradiasi tidak berbeda nyata dengan kontrol dalam meningkatkan kolonisasi akar, panjang akar dan bobot akar sorgum, namun iradiasi Gamma cenderung memperlihatkan hasil terbaik. Perlakuan komposisi media zeolit dan arang sekam secara umum tidak memberikan perbedaan yang nyata, sehingga produksi inokulan FMA dapat menggunakan berbagai komposisi sesuai dengan ketersediaan bahannya. Kata kunci : arang sekam, mikoriza arbuskula, sorgum, sterilisasi iradiasi, zeolit
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