Abstract

Serum alkaline phosphatase (ALP) levels increases in burn tissue damage. Several bioactive compounds found in Ashitaba, are expected to reduce serum ALP levels and enhance the process of wound healing. This study aims to prove that administration of oral Ashitaba extract can reduce serum ALP levels in rats exposed to burn trauma. This study uses true experimental post-test control group design with a total of 20 Sprague dawley rats as samples. All samples were inflicted with 2nd degree burn wound and divided into 2 groups, treatment group (Ashitaba extract 300 mg/KgBW) and control group. Blood serum were analyzed for ALP levels on the 2nd, 8th and 14th days. Kinetic-IFCC method was used to find serum ALP levels. Data was analyzed using Mann-Whitney Test, paired T-test amd independence T-test. Scalded wound size was measured macroscopically over the course of 21 days to find contracture rate. In conclusion, Ashitaba extract is not proven to significantly reduce the serum ALP levels, increase contracture rate and enhance burn wound healing process. However, there was slight increase in contracture rate in treatment group as compared to control group. In addition, there was a lower ALP levels in treatment group as compared to control group in day 8.

Highlights

  • Luka bakar didefinisikan sebagai kerusakan pada kulit dan/atau lapisan jaringan dalam yang disebabkan oleh api, cairan panas, gigitan beku, listrik, radiasi, laser dan bahan kimia

  • Serum alkaline phosphatase (ALP) levels increases in burn tissue damage

  • This study aims to prove that administration of oral Ashitaba extract can reduce serum ALP levels in rats exposed to burn injury

Read more

Summary

Introduction

Luka bakar didefinisikan sebagai kerusakan pada kulit dan/atau lapisan jaringan dalam yang disebabkan oleh api, cairan panas, gigitan beku, listrik, radiasi, laser dan bahan kimia. (RSCM) antara tahun 2012 dan 2016 menunjukkan bahwa penyebab utama luka bakar pada orang dewasa adalah api (53,1%), diikuti oleh air panas (19,1%), listrik (14%), kontak (5%) dan terakhir bahan kimia (3%) [1]. Penyebab utama luka bakar pada anak adalah air panas (52%), diikuti oleh api (26%), kontak (15%), listrik (6%) dan terakhir bahan kimia (1%). Sebuah artikel oleh GarciaEspinoza et al pada tahun 2017 menyebutkan bahwa penyebab luka bakar yang paling sering adalah karena cairan panas [2]. Terdapat tiga mekanisme utama dalam patofisiologi luka bakar yang meliputi trombosis akibat kerusakan pembuluh darah, peningkatan mediator inflamasi dan faktor proapoptosis. Isoenzim, alkalin fosfatase (ALP), yang biasanya hanya ditemukan pada organ tertentu, ditemukan meningkat pada kondisi patologis seperti kerusakan jaringan terutama pada inflamasi dan granulasi [6] [7]

Objectives
Methods
Results
Conclusion

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.