Abstract
OBJETIVOS: Foram avaliados os efeitos do uso de células tronco da medula óssea (CTM) e do plasma rico em plaquetas (PRP) na regeneração de nervos periféricos, utilizando um modelo estabelecido de regeneração de nervo ciático em ratos. MÉTODOS: Um defeito nervoso de 10 mm foi reconstruído com a utilização de um tubo de silicone preenchido com CTM, PRP ou ambos. O grupo controle recebeu somente o tubo de silicone. Foi realizado ainda um quinto grupo no qual o intervalo foi reconstruído utilizando o segmento ressecado do nervo. A função motora foi testada seis semanas após a cirurgia utilizando teste de marcha. Após o teste motor, os ratos foram anestesiados, o nervo ciático e o tubo foram ressecados e foi realizada microscopia eletrônica de transmissão. RESULTADOS: A análise quantitativa demonstra uma melhora na recuperação funcional no grupo CTM em comparação com os demais grupos. Regeneração nervosa foi demonstrada no grupo CTM por microscopia eletrônica de trasmissão com uma recuperação praticamente completa da anatomia neural. CONCLUSÃO: Nossos resultados sugerem que o uso de CTM associado com a técnica de tubulização promove uma satisfatória recuperação da função motora e regeneração nervosa.
Highlights
Autologous nervous graft is a treatment alternative for nervous injuries in which primary repair cannot be made without using excessive tension, some difficulties exist in using this technique, such as the adjustment of graft’s length and diameter
The objective of the present study was to assess the effect of bone marrow cells, platelet-rich plasma, and the combination of both on peripheral nerves regeneration compared to techniques currently in use for peripheral nerves injuries treatment
empty tube (ET): Empty Tube; marrow stem cells (MSC): Stem cells derived from Bone Marrow, platelet-rich plasma (PRP): Platelet-Rich Plasma; AG: Autograft
Summary
Autologous nervous graft is a treatment alternative for nervous injuries in which primary repair cannot be made without using excessive tension, some difficulties exist in using this technique, such as the adjustment of graft’s length and diameter. Numerous attempts have been made intending to induce nervous regeneration in nerve defects using non-biological materials, such as silicone tubes . [1,2,3] non-biological materials’ ability to induce nervous regeneration has been considered inappropriate, and the development of artificial nerves combining cells, such as the Schwann’s cells[4,5] and neurothrophic factors[6,7] together with non-biological materials has currently been broadly used. The objective of the present study was to assess the effect of bone marrow cells, platelet-rich plasma, and the combination of both on peripheral nerves regeneration compared to techniques currently in use for peripheral nerves injuries treatment
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