Abstract
This study aimed to evaluate the incubation performance, the Salmonella Enteritidis capacity of penetration through the eggshell and the ability of colonization of the gastrointestinal tract. Four hundred turkey eggs were incubated and distributed into four treatments with 100 experimental units each: CC and CCA (inoculation with placebo in eggshell and air chamber, respectively), IC and ICA (inoculation with 4.2 X 104 CFU/mL of Salmonella Enteritidis in eggshell and air chamber, respectively). The parameters of incubation were fertility, total hatchability and hatchability of fertile eggs, and relative chick weight to egg weight. Salmonella was investigated in shell, membrane, albumen/yolk and embryo of two eggs per treatment at one, seven, 14, 21 and 28 days. After birth, the frequency of recovery of the pathogen in meconium of all birds was determined. The variables were analyzed by ?-square test (?²) and Fischer test. During the whole incubation period, the agent has remained viable in 87.5% and 100% of eggshell samples in treatments IC and ICA, respectively. There was migration into eggs in 33.33% and 95.45% of the samples in treatments IC and ICA, respectively. The parameters of incubation were not affected when the pathogen was inoculated in the eggshell. It was also observed that inoculation of Salmonella Enteritidis in air chamber determined low hatchability with higher early embryonic mortality (P <0.05) than control treatment (CCA). Furthermore, air chamber control treatment determined low hatchability (P <0.05) with increase in late embryonic mortality (P<0.05) comparing to inoculation in eggshell. The intestinal colonization by the pathogen occurred in chicks from experimental inoculation in eggshell. It can be concluded that the assessment of fertility, hatchability and ratio of chick weight by egg weight does not show presence of Salmonella Enteritidis in the hatchery. However, hatchery contamination may be determined by detection of Salmonella Enteritidis in egg components and meconium. The method of inoculation via air chamber affected hatchability and embryo mortality. KEYWORDS: bacterial isolation; experimental inoculation; Meleagridis gallopavo ; salmonellosis.
Highlights
This study aimed to evaluate the incubation performance, the Salmonella Enteritidis capacity of penetration through the eggshell and the ability of colonization of the gastrointestinal tract
It was observed that inoculation of Salmonella Enteritidis in air chamber determined low hatchability with higher early embryonic mortality (P
The intestinal colonization by the pathogen occurred in chicks from experimental inoculation in eggshell
Summary
This study aimed to evaluate the incubation performance, the Salmonella Enteritidis capacity of penetration through the eggshell and the ability of colonization of the gastrointestinal tract. Four hundred turkey eggs were incubated and distributed into four treatments with 100 experimental units each: CC and CCA (inoculation with placebo in eggshell and air chamber, respectively), IC and ICA It was observed that inoculation of Salmonella Enteritidis in air chamber determined low hatchability with higher early embryonic mortality (P
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
