Abstract
The aim of this study was to verify the effect of increasing soybean oil levels and training on the enzyme activity of Aspartate Aminotransferase (AST) and Creatine kinase (CK) as indicative of muscle work. Experimental diets were composed by five soy oil levels in the concentrate (control- no oil, 6, 12, 18 and 24%). Twenty Arabian horses, male and female, average weight of 400 kg were submitted to an 80-km endurance race in a treadmill. The treadmill experiment was conducted in the Exercise Physiology Laboratory. The endurance simulation was divided in 4 phases of 20 km each. The effect (p < 0.05) of soybean oil addition and run distance for CK and AST variables during the effort test and also in the recovering time were observed. It was concluded that soybean oil presents a beneficial effect on muscle work, as it decreases the activity of AST and CK enzymes in diets with more of 6% of soybean oil in the concentrate. The elevated value showed was not necessarily linked to muscle pathology presence. Each animal must be analyzed individually, based on its corresponding basal enzymatic activity.
Highlights
Effect of diets with soybean oil addition and training over muscular activity of equines submitted to resistance test
The aim of this study was to verify the effect of increasing soybean oil levels and training on the enzyme activity of Aspartate Aminotransferase (AST) and Creatine kinase (CK) as indicative of muscle work
Experimental diets were composed by five soy oil levels in the concentrate
Summary
Foram utilizados 20 equinos da raça Árabe, machos e fêmeas, com idade média de 9,5±5,5 anos e peso vivo médio de 400 kg, submetidos a teste de esforço de 80 km em esteira rolante. Os animais foram adaptados às dietas experimentais, formuladas segundo o National Research Council (NRC, 1989) para equinos de esporte de média intensidade; também foram adaptados ao treinamento, baseado no limiar anaeróbico, determinado individualmente pelo método do limiar anaeróbico individual descrito por. Os equinos foram treinados seis vezes por semana, sendo três dias em esteira rolante, dois dias montados e um dia no redondel. O treinamento em esteira consistiu de um protocolo de treinamento aeróbico crescente, conforme avaliação pelo método do limiar anaeróbico individual, que englobava todas as andaduras, sendo o animal aquecido a passo e trote, submetido a galope e desaquecido a trote e passo. As dietas experimentais foram compostas por cinco concentrados, formulados com cinco níveis de inclusão de óleo de soja (controle-sem adição de óleo, seis, 12, 18 e 24%) e feno de Tifton 85, mantendo-se a relação de concentrado: volumoso de 50: 50 (Tabelas 1 e 2).
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