Efeito de Bacillus sp. e Trichoderma sp. no crescimento micelial de Sclerotium rolfsii

Abstract

This research aimed to evaluate the antagonistic effect of bacterial isolates of the genus Bacillus sp. and the fungus Trichoderma sp. on the mycelial growth of Sclerotium rolfsii, causal agent of sclerotia rot in plants Vigna unguiculata (L.) Walp. (cowpea). The assay was conducted using the crop pairing technique. The pathogen was obtained by the isolation of V. unguiculata (L.) Walp. with symptoms of the disease. The antagonists used were: Bacillus subtilis (LCB 30), B. subtilis (LCB 45), Bacillus sp. (BMH), Bacillus sp. (INV) and Trichoderma sp. Evaluations of mycelial growth were made when the entire surface of the culture medium was presented BDA-colonized by S. rolfsii in the treatment control (pathogen grown in the absence of antagonist). For the analyzes, a completely randomized design with five replications was used. The isolates B. subtilis (LCB30), Bacillus sp. (INV) and Trichoderma sp. They are capable of inhibiting fungus mycelial growth of S. rolfsii by reducing the fungal colony on 53.77%, 29.22% and 34.00%, respectively, with isolated B. subtilis (LCB45) and Bacillus sp. (BMH), inhibited 13.55% and 21.55% growth S.rolfsii, respectively, did not differ statistically from the control.