Efectos del sevoflurano en la lesión pulmonar inducida por la ventilación mecánica en un modelo experimental de pulmón sano

Abstract

Introduction and objectiveVentilator-induced lung injury (VILI) causes a systemic inflammatory response in tissues, with an increase in IL-1, IL-6 and TNF-α in blood and tissues. Cytoprotective effects of sevoflurane in different experimental models are well known, and this protective effect can also be observed in VILI. The objective of this study was to assess the effects of sevoflurane in VILI. Material and methodsA prospective, randomized, controlled study was designed. Twenty female rats were studied. The animals were mechanically ventilated, without sevoflurane in the control group and sevoflurane 3% in the treated group (SEV group). VILI was induced applying a maximal inspiratory pressure of 35cmH2O for 20min without any positive end-expiratory pressure for 20min (INJURY time). The animals were then ventilated 30min with a maximal inspiratory pressure of 12cmH2O and 3cmH2O positive end-expiratory pressure (time 30min POST-INJURY), at which time the animals were euthanized and pathological and biomarkers studies were performed. Heart rate, invasive blood pressure, pH, PaO2, and PaCO2 were recorded. The lung wet-to-dry weight ratio was used as an index of lung edema. ResultsNo differences were found in the blood gas analysis parameters or heart rate between the 2 groups. Blood pressure was statistically higher in the control group, but still within the normal clinical range. The percentage of pulmonary edema and concentrations of TNF-α and IL-6 in lung tissue in the SEV group were lower than in the control group. ConclusionsSevoflurane attenuates VILI in a previous healthy lung in an experimental subclinical model in rats.