EF-G-catalyzed translocation of anticodonstem–loop analogs of transfer RNA in the ribosome

Abstract

Corrigendum15 September 1998free access EF-G-catalyzed translocation of anticodonstem–loop analogs of transfer RNA in the ribosome Simpson Joseph Simpson Joseph Search for more papers by this author Harry F. Noller Harry F. Noller Search for more papers by this author Simpson Joseph Simpson Joseph Search for more papers by this author Harry F. Noller Harry F. Noller Search for more papers by this author Author Information Simpson Joseph and Harry F. Noller The EMBO Journal (1998)17:5519-5519https://doi.org/10.1038/sj.emboj.7591244 This article corrects the following: EF-G-catalyzed translocation of anticodon stem–loop analogs of transfer RNA in the ribosome15 June 1998 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The authors apologise for an error in Figure 1C. The arrows pointing to the toeprint positions were off by one position. The corrected figure below shows the correct toeprint positions. Figure 1.Construction of translocation complexes and toeprinting reactions. (A) Pre-translocation complexes contained 70S ribosomes programmed with gene 32 mRNA containing deacylated tRNAfMet in the P site and an ASL in the A site. Toeprinting was carried out by extending the 5′ 32P-labelled primer (AL2) with reverse transcriptase (RT). (B) EF-G-dependent translocation positions the UUU codon and its bound ASL in the P site. Toeprinting results in a product that is shorter by 3 nucleotides compared with that of the pre-translocation complex. (C) Sequence of gene 32 mRNA. Arrows indicate positions of reverse transcriptase stops when the P site is occupied by tRNAf Met or tRNAPhe, respectively. The dotted arrow indicates a weaker stop in pre-translocation complexes containing tRNAfMet and tRNAPhe. SD, Shine–Dalgarno sequence. Download figure Download PowerPoint Previous ArticleNext Article Volume 17Issue 1815 September 1998In this issue FiguresRelatedDetailsLoading ...