Een nieuwe methode voor de bepaling van de vatbaarheid van roggeplanten voor aantasting door stengelaaltjes (Ditylenchus dipsaci (Kühn) Filipjev)

Abstract

When large numbers of rye seedlings were grown in boxes of soil and inoculated with stem eelworms by means of a flat needle, to test their susceptibility to eelworm attack, the method proved unsatisfactory. Better results were achieved by a method described in the foregoing article in which rye seedlings were grown on thick filter paper pads; the grains being inserted into slits cut in the pad by means of a chisel (fig. 1). Each pad is covered with some sheets of thin filter paper on the side where the seedlings appear and the whole is dipped in Knopp's plant culture solution made up in 0.05 per cent. Aretan as a protection against the growth of bacteria and fungi. It is then put into an aluminium holder and placed vertically in a box with other pads which are watered daily or every second day. A trace of ferric chloride (FeCl3) is added to the water in order to prevent chlorosis of the seedlings which otherwise become evident about two weeks after planting. By numbering the pads and the slits in each, data can be obtained concerning each individual plant without risk of confusion (fig. 5).