Abstract
Urease immobilized into poly(vinylpyridine-co-styrene) film on the surface of ion sensitive field effect transistor (ISFET) has been used for determination of chelating agents (sequestrants). These species, particularly EDTA, restored catalytic activity of the enzyme if it was inhibited with cupric ions. Enzyme immobilization into a positively charged polymer seems to cause an increase of the EDTA restoration effect because of anion accumulation in the polymeric matrix. The resulting biosensor can measure EDTA concentration in the range of 5–500 ppm. The influence of other sequestrants (citrate and phosphate) were also examined.
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