EDNA Inactivation and Biofilm Inhibition by the PolymericBiocide Polyhexamethylene Guanidine Hydrochloride (PHMG-Cl).

Olena V Moshynets,Andrew J Spiers,Ianina Pokholenko,Nadiia P Kysil,Kateryna L Rudnieva,Geert Potters,Sergiy P Rogalsky,Taras P Baranovskyi,Viktoria V Potochilova,Ivan V Semenyuta,Oksana P Tarasyuk,Olga S Iungin,Svitlana Y Rymar,Larysa O Metelytsia

doi:10.3390/ijms23020731

Abstract

The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here, we investigated how different biocides affect the release of eDNA from mature biofilms of two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles. The effect of four groups of biocides, alcohols, hydrogen peroxide, quaternary ammonium compounds, and the polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl), was evaluated using PA and SA biofilms. Most biocides, except for PHMG-Cl and 70% ethanol, caused substantial eDNA release, and PHMG-Cl was found to block biofilm development when used at concentrations of 0.5% and 0.1%. This might be associated with the formation of DNA–PHMG-Cl complexes as PHMG-Cl is predicted to bind to AT base pairs by molecular docking assays. PHMG-Cl was found to bind high-molecular DNA and plasmid DNA and continued to inactivate DNA on surfaces even after 4 weeks. PHMG-Cl also effectively inactivated biofilm-associated antibiotic resistance gene eDNA released by a pan-drug-resistant Klebsiella strain, which demonstrates the potential of a polymeric biocide as a new surface-active agent to combat the spread of antibiotic resistance in hospital settings.

Highlights

The risk of multi-drug and pan-drug-resistant bacterial infections during or after a hospital stay is increasing worldwide
To investigate how much eDNA could potentially be released from biofilm contamination likely to be found in hospital environments following treatment with biocides, we used dehydrated 5-day-old Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus
The water-only treatment might release some DNA from cells that survived the preparation of the dehydrated samples through osmotic shock, but it is more likely that in this control, DNA is solubilized from cell debris and the original eDNA of the biofilm

Summary

Introduction

The risk of multi-drug and pan-drug-resistant bacterial infections during or after a hospital stay is increasing worldwide. Each drugresistant bacterium can contribute antibiotic resistance genes (ARGs), or resistome, to the hospital environment. This includes ARGs located within bacterial genomes Microbial eDNA is ubiquitous and can be found everywhere where microbial life is present. It is released by the process of cell death and lysis and may be secreted by living cells, but is not enclosed in living cells, may not remain with the cells from which it originated, and may persist for some time before it is degraded [1]. ARGs are an important source for naturally occurring HGT [9,10,11]

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