Abstract
The authors studied close to 2000 patients without known prostate cancer, each of whom had urine collected on first void after prostate biopsy. Their collection protocol differs from the more standard practice of collecting urine for PCA3 testing after an attentive digital rectal examination (DRE) or prostate massage. In theory, sampling urine after prostate manipulation—whether by DRE, attentive DRE, or transrectal ultrasonography and biopsy—allows for a comprehensive assessment of the prostate as such urine samples would be enriched in molecules derived from various parts of the prostate, even those not sampled by biopsy. In practice, however, there have been limited data using postbiopsy urine for molecular urinalysis, and one might assume such urine would be enriched in material originating from the sites of needle trauma to the prostate (and sampled pathologically). What literature there is does support a correlation between post-DRE sampling and postbiopsy sampling for molecular urinalysis, 1 Rogers C.G. Gonzalgo M.L. Yan G. et al. High concordance of gene methylation in post-digital rectal examination and post-biopsy urine samples for prostate cancer detection. J Urol. 2006; 176: 2280-2284 Abstract Full Text Full Text PDF PubMed Scopus (40) Google Scholar and the present manuscript represents a larger validation of this practice, demonstrating that postbiopsy urine sampling provides PCA3 results comparable with those in PCA3 studies that used post-DRE urine. Reflex PCA3 Messenger Ribonucleic Acid Testing: Validation of Postbiopsy Urine Samples and Correlation With Prostate Biopsy Findings in ∼2000 PatientsUrologyVol. 84Issue 5PreviewTo validate post-transrectal ultrasonography (TRUS) prostate biopsy (bx) urine samples for PCA3 messenger ribonucleic acid testing, including correlation of PCA3 score with concurrent bx findings. Full-Text PDF ReplyUrologyVol. 84Issue 5PreviewWe currently do not know the precise source of cells for the overexpression of PCA3 messenger ribonucleic acid in urine, and studies originally showing a correlation between higher PCA3 scores and biopsy detection of prostate carcinoma (CaP) may have led to the likely oversimplified concept that foci of frankly invasive cancer within the stroma are the source of elevated urinary PCA3. Full-Text PDF
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