Abstract
Anthocyanin hyperaccumulation is an important agricultural trait, associated with resistance to abiotic stress, pests, phytopathogenic fungi and bacterial diseases.B. napuswith increased anthocyanin pigmentation can be generated by genome editing. Many transcription factors of the MYB family are involved in stress response and anthocyanin biosynthesis. GenesAtMYB60, AtCPCandAtMYBL2are negative regulators of anthocyanin biosynthesis inArabidopsis, therefore the knockout of these genes can result in increased anthocyanin pigmentation. gRNA spacers were synthesized to target the orthologs of these genes, identified inBrassica napus. Resulting genetic constructs were introduced to the plant tissues by agroinfiltration. Transient expression of gRNAs targeting DNA-binding domains of MYB transcription factors along with Cas9 nuclease successfully promoted anthocyanin hyperaccumulation. These genetic constructs can be used for genome editing and production of new colored and stress tolerant varieties of oilseed rape.
Highlights
IntroductionBrassica napus L. (oilseed rape, rapeseed) is one of the most important crops in the world
Brassica napus L. is one of the most important crops in the world
In this study we demonstrate that transient knockout of MYB60, CPC and MYBL2 genes in the leaves of B. napus can promote anthocyanin pigmentation
Summary
Brassica napus L. (oilseed rape, rapeseed) is one of the most important crops in the world. (oilseed rape, rapeseed) is one of the most important crops in the world. It is used as a source of vegetable oil and protein, animal feed and biodiesel. Rapeseed is a good cover crop [1]. Canola, which is the rapeseed with low levels of erucic acid, belongs to the ‘big four’ biotech crops. High oleic acid canola is the only alternative variety. Wide use of pesticides brings harm to bees and other important organisms and promotes the development of pesticide-tolerance in weeds and pests. There have never been an attempt to enhance natural stress response mechanisms of B. napus on molecular level, it is a more effective and environmentally friendly method to increase the yields
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