Edible Safety Assessment of Genetically Modified Rice T1C-1 for Sprague Dawley Rats through Horizontal Gene Transfer, Allergenicity and Intestinal Microbiota.

Kai Zhao,Wei Shi,Fangfang Ren,Qiwen Liu,Peng Li,Jian Zhang,Hong Zhu,Fangting Han,Xiao Wu,Xueming Tang,Jianjun Lv,Guogan Wu,Yan Xu,Xiao Zhao,Jinbin Wang,Odir Antônio Dellagostin

doi:10.1371/journal.pone.0163352

Abstract

In this study, assessment of the safety of transgenic rice T1C-1 expressing Cry1C was carried out by: (1) studying horizontal gene transfer (HGT) in Sprague Dawley rats fed transgenic rice for 90 d; (2) examining the effect of Cry1C protein in vitro on digestibility and allergenicity; and (3) studying the changes of intestinal microbiota in rats fed with transgenic rice T1C-1 in acute and subchronic toxicity tests. Sprague Dawley rats were fed a diet containing either 60% GM Bacillus thuringiensis (Bt) rice T1C-1 expressing Cry1C protein, the parental rice Minghui 63, or a basic diet for 90 d. The GM Bt rice T1C-1 showed no evidence of HGT between rats and transgenic rice. Sequence searching of the Cry1C protein showed no homology with known allergens or toxins. Cry1C protein was rapidly degraded in vitro with simulated gastric and intestinal fluids. The expressed Cry1C protein did not induce high levels of specific IgG and IgE antibodies in rats. The intestinal microbiota of rats fed T1C-1 was also analyzed in acute and subchronic toxicity tests by DGGE. Cluster analysis of DGGE profiles revealed significant individual differences in the rats' intestinal microbiota.

Highlights

Rice (Oryza sativa L.) is one of the most important cereal crops and represents approximately 23% of all calories consumed worldwide [1]
Assessment of the safety of transgenic rice T1C-1 expressing Cry1C was carried out by: (1) studying horizontal gene transfer (HGT) in Sprague Dawley rats fed transgenic rice for 90 d; (2) examining the effect of Cry1C protein in vitro on digestibility and allergenicity; and (3) studying the changes of intestinal microbiota in rats fed with transgenic rice T1C-1 in acute and subchronic toxicity tests
It indicated that cry1C in genetically modified (GM) rice T1C-1 did not transfer to rat tissues

Summary

Introduction

Rice (Oryza sativa L.) is one of the most important cereal crops and represents approximately 23% of all calories consumed worldwide [1]. Extensive cultivation of modern high yielding varieties of crops has resulted in a significant increase in the yield of most food crops, including. Edible Safety Assessment of Genetically Modified Rice T1C-1 http://www.stcsm.gov.cn/. The Science and Technology Development Programme of Shanghai Acedemy of Agricultural Sciences,2012(8), http://www.saas.sh. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

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Conclusion