Abstract
Conventional growth media for microbes contains lots of non-edible components that are harmful to consume when the organism is ready to intake—like probiotics growing in chemical media (Lactobacillus grown in MRS media). The study was conducted to develop an edible and low-cost growth media that supports the growth of probiotics Lactic Acid bacteria for the enhancement of probiotic research. The 04 isolates of Lactobacillus (L. plantarum, L. rhamnosus, L. ferciminis and L. bifarmentans) and 02 Bifidobacterium (B. infantis and B. Bifidum) were used for the evaluation of medium efficacy. To formulate the edible culture media, 04 vegetables and 02 pulses were used. The media was formulated in different formulations. For Lactobacillus, maximum growth was observed at MM-2 media formulation that was about 15.62 log CFU/ml for L. plantarum and compared to MRS media that was 11.78 log CFU/ml. Bifidobaterium showed the highest viability at MW-1 edible media formulation which was about 12.72 log CFU/ml whereas in Bifidobacteria selective media the cell viability was 12.48 log CFU/ml. The edible media has no toxic or unhealthy effect on mice while trailing in an animal model and shows excellent results in encapsulation with alginate. In comparison with the performance of traditional chemical media, the formulated media were found to be cost-effective and safe for human consumption.
Highlights
IntroductionThe organisms are separated from media and introduced into fermentation
Some are made by cooking milk or meat products in acid, but most are made by incubating milk or meat with trypsin, pepsin, or other proteolytic enzymes to digest the protein to a mixture of amino acids, peptides, and polypeptides [7] [8] [9]
Lactobacillus are often used as probiotics, in dairy food products that are grown in mainly De Man Rogosa and Sharpe Agar (MRS agar) which contains manganese sulfate and magnesium sulfate
Summary
The organisms are separated from media and introduced into fermentation For this reason, the constituents of the media are very important as, if it contains any component that is harmful to human, it may come across the fermented food through the starter culture. Lactobacillus are often used as probiotics, in dairy food products that are grown in mainly De Man Rogosa and Sharpe Agar (MRS agar) which contains manganese sulfate and magnesium sulfate. These components can cause irritation in skin, respiratory system and in long term repeat it may cause organ damage [13]
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