Abstract
This study is focused on the detection of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) mRNA expression levels and protein positions in seven stages of cashmere goat fetus development (45, 55, 65, 75 95, 115, and 135 d), with the main goal of investigating the effect of EDA and EDAR on genes related to hair follicle development. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to measure EDA and EDAR expression levels in seven stages of cashmere goat fetus development. Immunohistochemistry (IHC) was used to locate EDA and EDAR in the critical stage of fetal hair follicle development (45–135 d). EDA and EDAR expression in fetal fibroblasts and epithelial cells was interfered with by short hairpin RNA (sh-RNA). The results indicated that EDA and EDAR were both expressed in the skin tissue in the seven cashmere goat embryo stages. Moreover, EDA and EDAR play an important role in the formation of embryonic placode (Pc). After interfering with EDA and EDAR, the expression of BMP2, BMP4, noggin, -catenin, TGF-2, Wnt-10b, and NOTCH1 in fibroblasts and epithelial cells changed significantly. This study provides a theoretical and experimental basis for further studying the molecular regulation mechanism of hair follicle development.
Highlights
The Inner Mongolian cashmere goat is an important source of germplasm with high economic value
Ment of hair follicles, this process is active in a large number of signalling pathways, such as the Wnt/β-catenin signalling pathway, TGF-β signalling pathway, and mitogen-activated protein kinase (MAPK) signalling pathway
We found that ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) were expressed in the Pc during the seven fetal stages of Inner Mongolian cashmere goats
Summary
The Inner Mongolian cashmere goat is an important source of germplasm with high economic value. In the developmental process of the formation of the structure of cashmere goat hair follicles, we conducted a morphogenesis study and found that on gestational day 45, fetal epidermis was the only layer of flat cells that did not form primary follicles (Yin et al, 2005). Most secondary follicles develop their complete structure by day 125 of fetal growth, and hair matrix cells surround the dermal papillae. Both dermal papillae and primary hair follicles have an oval shape, which facilitates the penetration of villi through the body surface. RNA interference was used to explore its effect on genes related to hair follicle development through interference with fetal fibroblasts and epithelial cell EDA and EDAR expression, providing a theoretical and experimental basis for exploring the molecular mechanism of hair follicle development
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