Abstract
The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the GhLETM1 gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the GhLETM1 gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of GhLETM1. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties.
Highlights
Using cytoplasmic male sterile lines to cultivate hybrid varieties in crops can produce great economic and social benefits
Further sequence alignment and comparative analysis of leucine zipper/EF-hand-containing transmembranes (LETMs) among different plant species based on amino acid sequences are consistent with and extend the report above (Figure 1)
With the hypothesis of a strict and sensitive expression dose effect of the LETM gene and corresponding precise control on stamen development, the investigation purpose of the current study is to focus on the ectopic expression dose effect of LETM on plant stamen development and male fertility
Summary
Using cytoplasmic male sterile lines to cultivate hybrid varieties in crops can produce great economic and social benefits. It is of great theoretical and practical significant to mine new genes related to fertility and develop male sterility germplasm innovation in plants. Based on our preliminary mutant analysis earlier, we discovered that the leucine zipper/EF-hand-containing transmembrane (LETM) gene was associated with mutant phenotype of male sterility. The homologous LETMs have conserved sequences in lower eukaryotes, animals and plants [1]. Studies have shown that LETM mediated the regulation of mitochondrial ATP production and biogenesis [2], and that LETM knockdown or overexpression robustly increases or decreases the mitochondrial Ca2+ levels in cells, respectively [3].
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