Abstract
Phytochromes sense red/far-red light and trigger a cascade of physiological responses in plant. Here, a phytochrome B homolog, GmPHYB1, was amplified from the soybean genome, and its expression profiles were obtained for various parts of the plant and at various developmental stages. The gene was ectopically expressed in Arabidopsis thaliana, driven by CaMV 35S promoter, to study the physiological functions of the gene product. The overexpressors of GmPHYB1 behaved similarly to those of AtPHYB, but with some subtle differences with respect to the acceleration of flowering under short day conditions and the growth of the hypocotyl under certain light fluence rate. The results suggested that this soybean PHYB homolog was well conserved both at the level of sequence and physiological function.
Highlights
For better growth and development, plants have evolved a series of photoreceptors to respond to light
PHY molecules are composed of a linear tetrapyrrole chromophore covalently linked to an apoprotein and function as dimers that undergo a reversible comformational change between the inactive (Pr) and the active (Pfr) form in response to red or far-red light, allowing the phytochrome to act as a switch that is turned on or turned off [4,5,6]
Its sequence was used to design a pair of primers (Table S1) to amplify the soybean PHYB gene from a leaf mRNA template extracted from the soybean cultivar Kennong 18
Summary
For better growth and development, plants have evolved a series of photoreceptors to respond to light. In A. thaliana, PHYB is the principal and primary mediator of red light responses and shade avoidance [8] It predominates in lightgrown plants [9], promoting seed germination and de-etiolation in response to red light, inhibiting shade avoidance responses under a high ratio of red: far-red light (R:FR) [10]. Such responses are important for the plants to survive in the ever-changing environmental conditions during its life cycle
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