Abstract
Cadmium (Cd) is one of the most toxic heavy metals for plant growth in soil. ATP-binding cassette (ABC) transporters play important roles in biotic and abiotic stresses. However, few ABC transporters have been characterized in poplar. In this study, we isolated an ABC transporter gene PtoABCG36 from Populus tomentosa. The PtoABCG36 transcript can be detected in leaves, stems and roots, and the expression in the root was 3.8 and 2 times that in stems and leaves, respectively. The PtoABCG36 expression was induced and peaked at 12 h after exposure to Cd stress. Transient expression of PtoABCG36 in tobacco showed that PtoABCG36 is localized at the plasma membrane. When overexpressed in yeast and Arabidopsis, PtoABCG36 could decrease Cd accumulation and confer higher Cd tolerance in transgenic lines than in wild-type (WT) lines. Net Cd2+ efflux measurements showed a decreasing Cd uptake in transgenic Arabidopsis roots than WT. These results demonstrated that PtoABCG36 functions as a cadmium extrusion pump participating in enhancing tolerance to Cd through decreasing Cd content in plants, which provides a promising way for making heavy metal tolerant poplar by manipulating ABC transporters in cadmium polluted areas.
Highlights
Cadmium (Cd) is a highly toxic pollutant in the environment
The results showed that overexpressing PtoABCG36 is effective in enhancing Cd tolerance through decreasing Cd content in plants, indicating that PtoABCG36 transporter functions as a cadmium extrusion pump to participate in Cd stress in plants, which provides a reasonable way to make heavy metal tolerant poplar by manipulating ATP-binding cassette (ABC) transporters in the areas with cadmium pollution
J.CMdol.inSci.P2t0o1A9,B2C0,G323963-carrying Δyap1 and Y252 was significantly less (52.5% and 20.3% le6sosf, 13 respectively) than that in mutant and wild-type. These results indicated that PtoABCG36 can contribute to Cd resistance by transporting it out of the yeast cells
Summary
Cadmium (Cd) is a highly toxic pollutant in the environment. Cadmium is nephrotoxic, and it can lead to serious human diseases, including kidney disorders, bone damage and neurotoxicity [1]. The results showed that overexpressing PtoABCG36 is effective in enhancing Cd tolerance through decreasing Cd content in plants, indicating that PtoABCG36 transporter functions as a cadmium extrusion pump to participate in Cd stress in plants, which provides a reasonable way to make heavy metal tolerant poplar by manipulating ABC transporters in the areas with cadmium pollution. J.CMdol.inSci.P2t0o1A9,B2C0,G323963-carrying Δyap and Y252 was significantly less (52.5% and 20.3% le6sosf, 13 respectively) than that in mutant and wild-type These results indicated that PtoABCG36 can contribute to Cd resistance by transporting it out of the yeast cells. Our study provided the evidence for the biological functions of PtoABCG36 as a transporter in regulating Cd resistance in plants It plays a crucial role in reducing Cd accumulation in plants, providing a theoretical basis to make heavy metal tolerant poplar by manipulating ABC transporters in cadmium polluted areas. The present study has provided insight on the roles of ABCG transporters in economic forest cultivation
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
