Abstract
Inositol polyphosphate 5-phosphatases (5PTases) function in inositol signaling by regulating the catabolism of phosphoinositol derivatives. Previous reports showed that 5PTases play a critical role in plant development and stress responses. In this study, we identified a novel 5PTase gene, Gs5PTase8, from the salt-tolerance locus of chromosome 3 in wild soybean (Glycine soja). Gs5PTase8 is highly up-regulated under salt treatment. It is localized in the nucleus and plasma membrane with a strong signal in the apoplast. Ectopic expression of Gs5PTase8 significantly increased salt tolerance in transgenic BY-2 cells, soybean hairy roots and Arabidopsis, suggesting Gs5PTase8 could increase salt tolerance in plants. The overexpression of Gs5PTase8 significantly enhanced the activities of catalase and ascorbate peroxidase under salt stress. The seeds of Gs5PTase8-transgenic Arabidopsis germinated earlier than the wild type under abscisic acid treatment, indicating Gs5PTase8 would alter ABA sensitivity. Besides, transcriptional analyses showed that the stress-responsive genes, AtRD22, AtRD29A and AtRD29B, were induced with a higher level in the Gs5PTase8-transgenic Arabidopsis plants than in the wild type under salt stress. These results reveal that Gs5PTase8 play a positive role in salt tolerance and might be a candidate gene for improving soybean adaptation to salt stress.
Highlights
Cultivated soybean (Glycine max) is an important economic crop as a main source of dietary protein and oil
The results showed that the expression of Gs5PTase8 was highly up-regulated, especially at 24 h after salt treatment in both leaves and roots, suggesting Gs5PTase8 would be involved in salt responses
The BLASTP searches showed that its closest homolog in Arabidopsis thaliana is At5PTase8, so the gene has been named as Gs5PTase8 (Figure 1B)
Summary
Cultivated soybean (Glycine max) is an important economic crop as a main source of dietary protein and oil. Its development and agricultural productivity could be severely restricted by salt stress [1,2]. Around 20% irrigated land is salt affected and the salt-affected regions continue to expand [3,4]. It is necessary to improve salt tolerance in cultivated soybean. Wild soybean (Glycine soja), the close related species of Glycine max, could confer higher tolerance to salinity or other stresses. Wild soybean could supply resistant gene resources for molecular breeding in soybean. It is meaningful to identify new salt-tolerance genes from Glycine soja and characterize their molecular mechanism
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