Abstract
Low-temperature stress severely affects the growth, development, and geographical distribution of various crop plants, resulting in significant economic loss to producers. In a quest to identify cold-regulated genes, we constructed a cDNA suppression subtractive library from a high altitude adapted ecotype of Lepidium. We cloned a cold-induced gene LlaCIPK from the subtracted cDNA library which gave homology to Arabidopsis CIPK15 gene. The predicted 3D structure of LlaCIPK protein also showed homology with Arabidopsis CIPK protein. Quantitative real-time PCR analysis in Lepidium seedlings exposed to 6 h of cold stress shows a 3-fold increase in the expression of LlaCIPK transcript. The expression of LlaCIPK was also differentially regulated by ethylene, CaCl2, ABA, and SA treatments. Ethylene and CaCl2 treatments up regulated LlaCIPK expression, whereas ABA and SA treatments down regulated the LlaCIPK expression. Transgenic plants overexpressing LlaCIPK gene under constitutive promoter show an increased level of proline and cell membrane stability. Taken together, our results suggest that the LlaCIPK contributes to the cold-response pathway in Lepidium plants.
Highlights
Due to their sessile nature, plants face various abiotic stresses during their lifespan
Our study shows that LlaCIPK participates in cold stress tolerance response and this gene could be utilized to potentially improve plant resistance to cold stress
Performance of the transgenic plants was scored in terms of survival rate by exposingdecide them to cold at 4 °Cthey for 7 days, followed by going back compels to the normal temperature
Summary
Due to their sessile nature, plants face various abiotic stresses during their lifespan. CIPK family has been reported to be regulated by stresses like drought, wounding, cold, salt as well as by ABA in many plant species [20,26,27,28,29,30]. Kim et al (2003) studied cipk during various abiotic stresses and showed that CIPK3 regulates the cold and ABA-induced expression of stress associated genes by positively mediating the Ca2+ signal [31]. PsCIPK and PsCBL coordinately up-regulated during the exposure of pea plants to NaCl, wounding and cold whereas drought and abscisic acid did not show any effect on the expression of these genes, suggesting the specificity of the CBL-CIPK expression pathway [32]. Our study shows that LlaCIPK participates in cold stress tolerance response and this gene could be utilized to potentially improve plant resistance to cold stress
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