Abstract
GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses.
Highlights
Peanut (Arachis hypogaea) is an important oilseed and food crop cultivated worldwide
A 1212 bp of full-length cDNA sequence of GLK1 was cloned from a cultivated peanut by reverse transcription PCR (RT-PCR) using specific primers
The CDS sequence was deposited to GenBank under accession number MK952147 and designated as AhGLK1b (A. hypogaea GOLDEN2-like 1 transcription factor)
Summary
Peanut (Arachis hypogaea) is an important oilseed and food crop cultivated worldwide. It is a major source of human nutrition, including oil and protein. Being important to both smallholders and large commercial producers, the peanut is widely grown in more than 100 countries every year in tropical and subtropical regions [1]. The peanut yield is decreased by a variety of pathogens including fungal, bacterial and viral pathogens. Most of the plant species are vulnerable to a variety of pathogens and pests including fungi, bacteria, oomycetes, nematodes, insects and viruses and in favorable conditions they turn into a disease. Due to preharvest pathogens and pests, an average of 26%
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