Abstract
There is a growing interest in plant microbiome’s engineering to optimize desired functions such as improved phytoremediation. This study is aimed at examining the microbial communities inhabiting the roots and rhizospheres of two Salix miyabeana cultivars that had been grown in a short-rotation intensive culture (SRIC) system for six years in a soil contaminated with the discharge from a petrochemical factory. DNA was extracted from roots and rhizospheric soils, and fungal ITS and bacterial and archaeal 16S rDNA regions were amplified and sequenced using Illumina MiSeq technology. Cultivars ‘SX61’ and ‘SX64’ were found to harbor a similar diversity of fungal, bacterial, and archaeal amplicon sequence variants (ASVs). As expected, a greater microbial diversity was found in the rhizosphere biotope than in the roots of both cultivars, except for cultivar ‘SX64’, where a similar fungal diversity was observed in both biotopes. However, we found that microbial community structures were cultivar- and biotope-specific. Although the implication of some identified taxa for plant adaptability and biomass production capacity remains to be explored, this study provides valuable and useful information regarding microbes that could potentially favor the implantation and phytoremediation efficiency of Salix miyabeana in mixed contamination sites in similar climatic environments.
Highlights
The microbiome, or to some extent, the phytomicrobiome, refers to the community of microorganisms that collectively colonized most parts of the plant, including the phyllosphere, rhizosphere, and endosphere [1]
The inner parts of the roots are frequently colonized by nonmycorrhizal ascomycetes, which are collectively referred to as dark septate endophytes (DSE) [9]
We examined the root and the rhizosphere microbiomes of two cultivars of Salix miyabeana (‘SX61’ and ‘SX64’) that were grown under short-rotation intensive culture (SRIC) for six years in a mixedcontaminated soil
Summary
The microbiome, or to some extent, the phytomicrobiome, refers to the community of microorganisms (i.e., fungi, bacteria, and archaea) that collectively colonized most parts of the plant, including the phyllosphere, rhizosphere, and endosphere [1]. The arbuscules and Hartig nets are effective interfaces to give fungi direct access to the plant’s carbohydrates Both AMF and EMF have extraradical mycelia with a great capacity to extend beyond the root zone, which greatly benefits the growth and health of the plant, allowing it to absorb a greater amount of water and nutrients, which would not be accessible otherwise [9]. The inner parts of the roots are frequently colonized by nonmycorrhizal ascomycetes, which are collectively referred to as dark septate endophytes (DSE) [9]. Even if their presence in plant roots has been known for over a century, their function and taxonomic affinities are still elusive [13]. Less is known about their relations with plants, it is assumed that they would interact positively due to their ubiquitous occurrence within the microbiome of healthy plants [16]
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