Ecotypic Differentiation in Morphology and Cold Resistance in Populations of Colobanthus quitensis (Caryophyllaceae) from the Andes of Central Chile and the Maritime Antarctic

Abstract

Colobanthus quitensis is one of only two vascular plant species that occur in the Antarctic islands. We evaluated morphological and physiological traits and survival after freezing in individuals of C. quitensis collected in two populations: one from the Andes of central Chile and the other from the maritime Antarctic. We addressed whether these populations are ecotypes in terms of shoot morphology and physiology, and cold resistance. We also evaluated genetic differentiation at the internal transcribed spacer (ITS) regions. Under controlled growing conditions, Andean plants had longer and narrower leaves and longer peduncles. However, shoot biomass was similar in both populations. There was a 1.17% sequence divergence of ITS regions between populations. Survival to freezing at temperatures from 0° to −16°C was not different in nonacclimated plants. After cold-acclimation at 4°C, freezing tolerance was greater in Antarctic plants. Both Andean and Antarctic plants increased soluble sugars with increasing acclimation time, but it did not differ between populations. Shoot water content decreased with acclimation time in both populations, but Antarctic plants maintained slightly higher water contents after cold exposure. Shoot growth did not differ during acclimation. The studied populations of C. quitensis represent different morphological and cold resistant ecotypes despite their genetic similarity. However, the mechanistic basis of the higher survival to freezing of Antarctic plants was not elucidated.