Ecotoxicity of polystyrene microplastics to submerged carnivorous Utricularia vulgaris plants in freshwater ecosystems

Abstract

Much attention is currently paid to microplastic (MP) pollution, particularly in marine systems. There is increasing concern regarding the potential toxicity of MPs to organisms at the physiological and morphological levels. However, little is known about the impact of MPs on aquatic life, despite their ubiquitous presence in freshwater ecosystems. In this study, the aquatic plant Utricularia vulgaris was exposed to 1, 2 and 5 μm polystyrene fluorescent MP particles at concentrations of 15, 70 and 140 mg/L for 7 days. The toxic effects of MPs on the growth rate and morphological and physiological characteristics of U. vulgaris were assessed. The results showed that the relative growth rates and the functional traits of leaves (morphological and photosynthetic) were significantly inhibited at a high concentration of MP particles (140 mg/L) when compared to the control group. The impacts on growth performance were likely due to bioaccumulation of MPs in the bladders, as shown by confocal microscopy. Furthermore, the antioxidative enzyme activities showed that high concentrations of MPs induce high ecotoxicity and oxidative damage to U. vulgaris. Thus, U. vulgaris has the potential to be an excellent bioindicator of MP pollution in freshwater ecosystems and should further be applied in ecological risk assessments of the effects of MPs on higher aquatic plants.