Ecosystem impact on fungal identification using sodium dodecyl sulfate polyacrylamide gel electrophoresis method (SDS-PAGE) technique

Abstract

Twenty isolates of Aspergillus species were collected from five different substrate sources and different ecosystems in Jeddah, Saudi Arabia which included agricultural soil, Wheat grain, Sewage dump, soil around car oil dump and Marine fauna. The isolates were cultivated on two different media (Czapek Dox and Waksman) for protein banding patterns determination. The total fungal proteins were analyzed using discontinuous sodium dodecyl sulfate polyacrylamide gel electrophoresis method (SDS-PAGE). The relationships among the studied isolates of Aspergillus species expressed by similarity coefficient have been demonstrated as phonogram, based on the analysis of the recorded attributes using NTSYS-pc version 2.02i. The study revealed that a wide variation in the protein patterns was observed between isolates grown on Czapek Dox’s medium and those grown on Waksman’s medium. Also, there is a remarkable correlation between sources of isolates and protein profiles of the studied isolates. Key words: Aspergillus, ecosystems, fungal proteins, sodium dodecyl sulfate polyacrylamide gel electrophoresis method (SDS-PAGE), Jeddah, Saudi Arabia.