Abstract
Industrial waste, such as crude glycerol, was used for vitamin K2 by B. subtilis Z-15. Crude glycerol could be used instead of pure glycerin for vitamin K2 production. The combination of soybean peptone and yeast extract was more conducive to the synthesis of vitamin K2. The optimal composition of medium was obtained by response surface methodology. The results indicated that the optimal medium was as follows: 6.3% crude glycerol, 3.0% soybean peptone concentration and 5.1 g/L yeast extract. Under the optimal culture medium, vitamin K2 production was increased to 45.11 ± 0.62 mg/L. The fermentor test further proved that the use of crude glycerol affected neither the synthesis of vitamin K2 nor the growth of B. subtilis. These investigations could lay a foundation for reducing the pollution of crude glycerol, exploring a late model for vitamin K2 cleaner production.
Highlights
Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body, and it is called menaquinone-n (MK-n, n = 1–14), where n denotes the number of isoprene units in the side chain[1,2]
Some scholars used response surface method (RSM) to optimise the medium to improve the yield of vitamin K2, there was no relevant report in the use of RSM to optimise the crude glycerol medium to produce vitamin K22
The effects of different carbon sources on vitamin K2 production were studied after shaking flask fermentation for 4 days
Summary
Bacillus subtilis has become the most important microorganism for vitamin K2 fermentation, due of its fast growth, easy cultivation, and high vitamin K2 content[7] It is one of the ideal strains for industrial production of vitamin K2. Hu et al.[9] reported that a vitamin K2 yield of 31.18 mg/L in Bacillus natto was achieved under optimal conditions containing 53.6 g/L glycerol and 100 g/L soy peptone It can be seen from the above studies that glycerol is used as a carbon source in many vitamin K2 studies. The utilisation of crude glycerol provides a carbon source for vitamin K2 production, which reduces the cost of the medium. This process can offset the disposal costs of crude glycerol. The goal was to investigate the feasibility of producing vitamin K2 from crude glycerol and to optimise the fermentation medium of B. subtilis Z-15 using RSM
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