Abstract
To investigate the simultaneous influence of different non-nutritional factors in production and physical-chemical characteristics of chitosan obtained by Syncephalastrum racemosum we used individually agroindustrial wastes as the only nutritional sources for fungus growth. The growth conditions were evaluated according to Factorial Design, 24 with three central points in order to determine the mainly factors for maximum production of microbiological chitosan in submerged culture. Syncephalastrum racemosum grown in corn steep liquor and yield up to 7.8 g chitosan/kg of substrate in the best condition by factorial design. The microbiological chitosan obtained has deacetilation degree 88.14%, crystallinity rate of 55.96%, mass decomposition process at 304.43 ºC, and low molecular weight. To fast production we performed a kinetic study and confirmed that at 36 h the chitosan production is higher and the physical-chemical characteristics were maintained. This research describes, for the first time, the factorial study of chitosan production by Syncephalastrum racemosum in agroindustrial wastes and its economic potential for commercialization.
Highlights
Nowadays, the natural synthesis of biopolymers by microorganisms has been related to selected strain, to lifecycle phase of the microorganism, to carbon and nitrogen sources of the culture medium, to pH and to incubation temperature
There are the chitosan (CS) a biopolymer constituted of β-(1-4)-2-amino-2deoxy-D-glucopyranose glycosidic residues, which is naturally found as constituent of the cell wall of fungi, especially the species belonging to the Zygomycetes class (CamposTakaki 2005, Muzzarelli & Peter 1997), or chemical obtained by deacetylation of chitin residues (Tajik et al 2008)
Among the Zygomycetes, especially, the Syncephalastrum racemosum specie has presented a great potential for chitosan production when it is grown in simple culture medium and of low cost (Amorim et al 2006)
Summary
The natural synthesis of biopolymers by microorganisms has been related to selected strain, to lifecycle phase of the microorganism, to carbon and nitrogen sources of the culture medium, to pH and to incubation temperature. All these factors can cause an increase and improvement of the physical-chemical properties of biopolymers (Amorim et al 2006, Fasciotti 2017, Mahopatra et al 2017, Silva et al 2010, Wang et al 2008). Due to the great interest in production of chitosan (Batista et al 2013, Dos Santos et al 2013, Zhang et al 2014) and biotechnological applications (Baldrick 2010, El-Sawy et al 2010, Ibrahim et al 2014) by scientific community, this research aimed at investigating different types of organic waste, temperature, pH and inoculum size as parameters which could influence microbiological chitosan production and its physical-chemical characterization
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
