Abstract
BackgroundTo investigate the ecological effects of chemical and biological control methods on tobacco wildfire disease, a plot field experiment was conducted to compare the control efficiency and mechanisms of a chemical pesticide (kasugamycin wettable powder, KWP) and a biological control agent (BCA) through high-throughput sequencing of bacterial 16S rRNA genes.ResultsThe results showed that the BCA displayed better performance in decreasing the disease index and morbidity of tobacco than the chemical pesticide. By monitoring the endophytic community within tobacco leaves, it was found that the control effects of these two methods might be mediated by different changes in the endophytic bacterial communities and community assembly patterns. The application of either method decreased the taxonomic diversity of the leaf endophytic community. Compared to the BCA, KWP showed a more significant effect on the endophytic community structure, while the endophytic community treated with the BCA was able to return to the original state, which presented much lower disease infection. The disease control efficiency of KWP and BCA treatments might be achieved by increasing the abundance of Sphingomonas and Streptophyta, respectively. Furthermore, an analysis of the ecological processes in community assembly indicated that the BCA strengthened the homogeneous and variable selection, while KWP enhanced ecological drift.ConclusionsThe results suggested different control mechanisms between KWP and BCA treatments, which will help in developing diverse ecological strategies for plant disease control.
Highlights
IntroductionTo investigate the ecological effects of chemical and biological control methods on tobacco wildfire disease, a plot field experiment was conducted to compare the control efficiency and mechanisms of a chemical pesticide (kasugamycin wettable powder, Kasugamycin wettable powder (KWP)) and a biological control agent (BCA) through high-throughput sequencing of bacterial 16S rRNA genes
To investigate the ecological effects of chemical and biological control methods on tobacco wildfire disease, a plot field experiment was conducted to compare the control efficiency and mechanisms of a chemical pesticide and a biological control agent (BCA) through high-throughput sequencing of bacterial 16S rRNA genes
The control efficiency of the BCA reached 67.99% based on the disease index, which was significantly higher than that of Kasugamycin wettable powder (KWP) (LSD test, P < 0.05)
Summary
To investigate the ecological effects of chemical and biological control methods on tobacco wildfire disease, a plot field experiment was conducted to compare the control efficiency and mechanisms of a chemical pesticide (kasugamycin wettable powder, KWP) and a biological control agent (BCA) through high-throughput sequencing of bacterial 16S rRNA genes. Tabaci is a typical, destructive leaf disease of tobacco [1]; a great amount of effort has been made to control it, including spraying chemical pesticides, selecting wildfire-resistant cultivars, and using microbial agents [2]. Many studies have reported that biological and chemical methods have different control efficiencies against plant disease [6, 7], but the mechanisms of different. An acute understanding of the mechanisms underlying these interactions is necessary, regardless of the method that is utilized
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