Ecological responses to simulated benthic‐derived nutrient subsidies mediated by omnivorous fish

Abstract

Summary1. Fish can play an important role in coupling benthic and pelagic habitats by consuming benthic prey and providing essential nutrients to algae in dissolved form. However, little is known about the factors affecting the magnitude of this nutrient subsidy.2. Using laboratory and mesocosm experiments we evaluated how varying ingestion rates of bluegill sunfish (Lepomis macrochirus) affects fish excretion rates of both nitrogen (N) and phosphorus (P). During the 10‐week mesocosm experiment, we also evaluated how varying ingestion rates may affect plankton community dynamics, and nutrient flux between pelagic and benthic habitats. Lastly, bioenergetic/mass balance models were used to examine the nutrient stoichiometry of fish body composition and excretion products.3. Under laboratory conditions, both N and P excretion rates increased with increased ingestion of benthic prey surrogates (earthworms). This effect was more pronounced for N than P. Furthermore, under the more realistic conditions of the mesocosm experiment ingestion rate had no significant effect on P excretion rate.4. Increased fish ingestion rate in the mesocosm experiment increased total algal biomass and the flux of nutrients from the water column to sediments. Effects of variable ingestion were much stronger on periphyton biomass and algal sedimentation rates than on phytoplankton or zooplankton biomass or composition.5. Fish body nutrient composition was greatly affected by ingestion rate. N content increased and P content decreased with ingestion rate. As a result, the N : P ratio of fish bodies also increased with ingestion rate. The N : P ratio of nutrients excreted by fish also increased with ingestion rate, counter to predictions of stoichiometric theory, which predicts that excreted N : P ratio is negatively correlated to body N : P. However, this finding can be explained by relaxing the assumption of constant nutrient assimilation rates, and our mass balance data suggest that assimilation rates vary indeed with ingestion rate.6. Our study provides experimental evidence that translocation of benthic‐derived nutrients by fish can affect the flux of nutrients among habitats, while also suggesting that stoichiometry models need to better incorporate how variable ingestion rates affect nutrient assimilation and excretion rates.