Ecological practices increase soil fertility and microbial diversity under intensive farming

Abstract

Intensive farming offers a potential solution to feed the growing population due to its high productivity. Conventional management (CO) based on inorganic fertilization practices degrades soil quality, but restorative practices including ecological intensification (EI) and organic management results in maintaining soil quality without compromising productivity. In this paper, two different management systems were evaluated: CO, based on inorganic fertilization, and EI, focused on providing organic nutrients to soils to support crops.EI increased soil fertility, together with higher alpha diversity indices, more differentially abundant amplicon sequence variant (ASVs) (247 EI vs. 165 CO) and indicator taxa (60 EI vs. 32 CO). Distinct bacterial taxa were associated with the different management systems, revealing their roles in soil processes and nutrient availability. In the CO treatment, indicator genera such as Nitrospira and Desulfarculaceae were linked to N fertilization and nitrite oxidation, while RB41 was associated with phosphorus availability. Ammoniphilus, PAUC26f, and BSV26 were also indicators of CO management. Conversely, EI treatment promoted bacteria involved in organic matter decomposition and nutrient cycling, such as Halomonas, Chryseolinea and Rhodobacteraceae. Gemmatimonas, Steroidobacter, Altererythrobacter, Acidibacter and Anseongella contribute to carbon and nitrogen cycling. Burkholderiaceae and Rhodopirellula play roles in phosphate solubilization and organic P mineralization, respectively. Numerous taxa with plant growth-promoting (PGP) attributes, such as BIrii41, Pseudomonas, and Lysobacter, were also identified as indicators of the EI treatment.EI associated bacteria were positively correlated with soil organic carbon contents, nitrates, and exchangeable bases, while negatively correlated with CO bacteria. A distance-based multivariate multiple regression (DistLM) demonstrated a strong relationship (r2 = 0.78) between soil physicochemical variables and bacterial community structure, with SOC explaining the most variations in the model. Other significant parameters included potassium (K), electrical conductivity (EC), and nitrates. The results suggest that EI promotes more sustainable soils in terms of fertility and microbial diversity.