Abstract
Unique ecological/habitat media derived from four phases of a municipal wastewater treatment plant revealed the highest diversity (2.55-2.86) and evenness (0.79-0.87) for the raw sewage (R) medium. Richness was, however, inoculum- and media-dependent hence inocula R and P recorded the highest counts on media A and F, respectively.
Highlights
The continuing need for robust, sustainable and reliable technologies that are characterized by minimal carbon emissions/footprints must be matched by an growing and sound knowledge base of the underpinning microbial communities
The PCR was made [13] with 25 μL reaction volumes on a Primus 96 Plus (MWG Biotech, Ebersberg, Germany) and amplicons (5 μL) visualised on 1.5% (w/v) agarose gels stained with SYBR Safe (Molecular Probes, Eugene, U.S.A)
The Shannon-Wiener diversity (2.7 ± 0.06) and evenness (0.8 ± 0.02) were highest after cultivation on the raw sewage medium with statistically significant (p=0.0009) decreases recorded (0.4, H’; 0.12, E) for the other three media (Figure 1). These findings probably related to the high nutrient availability in the raw sewage tank and decreased types/concentrations of the subsequent [18]
Summary
The continuing need for robust, sustainable and reliable (bio) technologies that are characterized by minimal carbon emissions/footprints must be matched by an growing and sound knowledge base of the underpinning microbial communities. Despite their analytical power/capacity, ecogenomic tools do not provide phenotypic or physiological characterization of novel and previously uncultivated microbial strains [1]. Despite the debate for/against cultivation, with/without molecular analyses [6,7], the approach has been exemplified by many researchers [1,4,8,9,10,11] who used different approaches, and remains essential to elucidate the physiological responses of process microbial communities for subsequent exploitation
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