Ecological improvement of antimony and cadmium contaminated soil by earthworm Eisenia fetida: Soil enzyme and microorganism diversity

Abstract

Vermiremediation on improvement of antimony (Sb) and cadmium (Cd) contaminated soil was less reported. In this study, earthworm Eisenia fetida was exposed into soil spiked with Sb and Cd and their mixture for 30 days, and then we measured multiple soil enzyme activities and bacteria communities via enzymatic reaction and high-throughput sequencing of 16 S rRNA genes. The results showed that Sb and Cd at high treatment levels inhibited the activities of urease, neutral phosphatase and protease significantly, but earthworm could promote the activities of urease and neutral phosphatase by 17.75%–121.91% and 1.46%–118.97%, respectively. However, earthworms inhibited catalase and had no effect on protease. The Geometric Mean Index suggested that earthworms led to a higher soil biochemistry function. According to a taxonomic analysis, bacterial community structure predominantly consisted of phylum Proteobacteria, Actinobacteria, Firmicutes, etc. and class Gammaproteobacteria, Actinobacteria, Alphaproteobacteria, etc.; furthermore, Pielou index and Shannon index (Alpha diversity in the habitat) indicated that bacteria diversity and evenness increased in the presence of earthworms. The heating map revealed that earthworms made genus Sphingomonas, Flavobacterium, etc. and species Sphingomonas jaspsi, Conexibacter, etc. dominate. Overall, earthworm is a suitable remediation species to improve the ecological function of heavy metal polluted soil. However, the specific mechanism and causal relationship of how earthworm to control enzyme activity and bacteria community remained to be explored.