Abstract
BackgroundThe cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. However, no transcriptome information is available for the cotton aphid and sequence data are scarce. Therefore, we obtained transcriptome data.ResultsTo facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX.ConclusionsOur results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species.
Highlights
Aside from direct damage, Aphis gossypii Glover is an important vector of many viral diseases in the early cotton season [1]
We identified six putative Hsp90 genes from the cotton aphid transcriptome (Figure 3)
Quantitative real-time polymerase chain reaction (PCR) validation of Heat shock proteins (Hsps) To elucidate the function of the Hsp genes identified, we focused on their expressions in different developmental stages
Summary
Aside from direct damage, Aphis gossypii Glover is an important vector of many viral diseases in the early cotton season [1]. Heat shock proteins (Hsps) play a vital role in dealing with environmental stress [7] These proteins function as molecular chaperones that maintain or return proteins to their functional state, prevent the indiscriminate aggregation of denatured proteins, and target unfolded or aggregated proteins for degradation or removal [8]. The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest.
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