Abstract
The present study aimed to assess the Bacillus cereus SDN1 native bacterium's ability to clean up contaminated or polluted water. The isolated bacterium was identified by its morphological and biochemical characteristics, which were then confirmed at the genus level. Furthermore, the isolated B. cereus (NCBI accession No: MW828583) was identified genomically by PCR amplifying 16 s rDNA using a universal primer. The phylogenetic analysis of the rDNA sequence was analyzed to determine the taxonomic and evolutionary profile of the isolate of the previously identified Bacillus sp. Besides, B. cereus and the bacterial consortium were treated using sewage wastewater. After 15 days of treatment, the following pollutants or chemicals were reduced: total hardness particles removal varied from 63.33 % to 67.55 %, calcium removal varied from 90 % to 93.33 %, and total nitrate decreased range from 37.77 % to 22.22 %, respectively. Electrical conductivity ranged from 1809 mS/cm to 2500 mS/cm, and pH values ranged from 6.5 to 8.95. The outcome of in-situ remediation results suggested that B. cereus has a noticeable remediation efficiency to the suspended particles. A root tip test was also used to investigate the genotoxicity of treated and untreated sewage-contaminated waters on onion (Allium cepa) root cells. The highest chromosomal aberrations and mitotic inhibition were found in roots exposed to contaminated sewage water, and their results displayed chromosome abnormalities, including disorganized, sticky chain, disturbed metaphase, chromosomal displacement in anaphase, abnormal telophase, spindle disturbances, and binucleate cells observed in A. cepa exposed to untreated contaminated water. The study can thus be applied as a biomarker to detect the genotoxic impacts of sewage water pollution on biota. Furthermore, based on an identified bacterial consortium, this work offers a low-cost and eco-favorable method for treating household effluents.
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