Eco-friendly oriented synthesis of CeO2 NPs corroborated over silica gel: Synthesis, description of distinctive features and the study of its antioxidant and anti-human colorectal cancer action

Abstract

This research, while making every endeavor to elaborate measures in supporting little ceria NPs, intends to keep their proper action, competence and capacity, at a greater stability of SiO2 assistance. In this process, we synthesized the ceria NPs instinctive in SiO2 utilizing a green procedure, where the external functional groups of SiO2 gel could be modified with Pistacia natural covering the aqueous extract used as the reducing/stabilizing factor to shape and arrange more moderate ceria NPs (CeO2 NPs-SiO2). We obtained these features of the newly made CeO2 NPs-SiO2 by employing such advanced analytic techniques as TEM, FE-SEM, EDS-elemental mapping and FT-IR analysis. These results show the well-dispersed spherical shapes of ceria nanoparticles with average sizes varying from 20 to 30 nm over the surface of silica gel in average. To realize the natural entailment, we utilized these substances in anticancer research projects to fight against colon cancer cell lines (HT-29 and Caco-2) using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) procedure. This was also carried out in a way similar to the approach adopted in the natural assessment of antioxidant features by the DPPH (2,2-diphenyl-1-picrylhydrazyl) approach. This material displayed its effective anticancer characteristics relied on the DPPH check's, notably IC50 value. We usually explored that the proportion of cancerous cell lines' cell livability reduced in the ceria nanoparticles-Silica NP in a dosage-based way. The MTT test generated the related IC50 figures on the two cell lines, being 264 μg/mL and 218 μg/mL in order. The transmission density rate was specified at 545 nm, demonstrating toxicity on usual cell line up to 2000 μg/mL, proving to be rather negligible.